addZeroTime
adds a zero timepoint to a specific treatment's data
subset.
Arguments
- data
A
SummarizedExperiment
object containing the experimental data.- condition
Character
string corresponds to one of the columns from the colData of SE object.- treat
Character
string specifying the treatment to which zero timepoint should be added.- zeroTreat
Character
string specifying the treatment representing the zero timepoint.- timeRange
Character
vector specifying the timepoints to include for the treatment.
Value
A SummarizedExperiment
object with the zero timepoint added to
the specified treatment's data.
Details
The function performs the following steps:
Subsets the data for the specified treatment and time range.
Subsets the data for the zero timepoint of the specified zero treatment.
Combines the assays from the treatment and zero timepoint subsets.
Updates the column data to reflect the combined treatment.
Returns a
SummarizedExperiment
object with the combined data.
Examples
library(SummarizedExperiment)
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# Load multiAssayExperiment object
data("dia_example")
# Get SummarizedExperiment object
se <- dia_example[["Phosphoproteome"]]
colData(se) <- colData(dia_example)
# Call the function
addZeroTime(se, condition = "treatment", treat = "EGF",
zeroTreat = "1stCrtl", timeRange = c("20min","40min", "6h"))
#> class: SummarizedExperiment
#> dim: 500 6
#> metadata(0):
#> assays(1): intensity
#> rownames(500): s1 s2 ... s499 s500
#> rowData names(7): UniprotID Gene ... Sequence site
#> colnames(6): FullProteome_EGF_0min_rep2 FullProteome_EGF_0min_rep3 ...
#> FullProteome_EGF_0min_rep1 Phospho_EGF_0min_rep1
#> colData names(6): sample treatment ... sampleType sampleName